Ex Parte Brown et al - Page 8

                Appeal 2007-2955                                                                              
                Application 10/190,425                                                                        

                were then gently washed off . . . Arrays were then fixed” (Belov 4484,                        
                paragraph bridging left- and right-hand col.).                                                
                29. According to Belov, “[t]he main strength of the [ ] Array is speed and                    
                extensive immunophenotyping, enabling pattern recognition using large                         
                arrays of microscopic antibody dots” (Belov 4488, left-hand col.).                            
                Chang                                                                                         
                30. Chang describes a device “compris[ing] a pattern or array of minute                       
                antibody-coated spots on the surface of a support.  Each antibody-coated                      
                spot is made up of antibodies of a different and distinct specificity.  The                   
                spots serve as tiny, specific immuno-absorbents of cells” (Chang, col. 1, ll.                 
                59-63).                                                                                       
                31. “The density of antibodies in the antibody-coated spots is related to                     
                the function of the spots as immunoadsorbents of cells” (Chang, col. 3, ll.                   
                9-11).  “The density required to generate a microscopically uniform layer of                  
                cells is probably greater than the density minimally sufficient for tight cell                
                binding” (id. at col. 3, ll. 27-30).  “The preferred concentration of antibodies              
                in the solution applied to the surface for coating is about 10 μg/ml” (id. at                 
                col. 3, ll. 56-58).                                                                           
                32. “Preferably, the support is a solid substance . . . such as a glass or                    
                plastic coverslip” and “[t]he antibodies in the antibody coated spots are                     
                conjugated to the surface of the support . . . either covalently or non-                      
                covalently” (Chang, col. 2, ll. 46-52).                                                       
                33. “Cells to be tested for the presence of certain surface antigens are                      
                placed in suspension in normal saline and applied to the surface of the                       
                device” (Chang, col. 4, ll. 18-31).                                                           


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