Appeal No. 93-2460
Application No. 07/590,647
APPENDIX
1. A method for producing a gene encoding a glyphosate-
tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme
which comprises substituting, in a EPSP synthase gene encoding a
protein containing the amino acid sequences shown below located
in the indicated positions, a codon encoding an alanine residue
for the second glycine residue in a first amino acid sequence:
-L-G-N-A-G-T-A-
located between positions 80 and 120 in the EPSP synthase gene,
and further substituting a codon encoding a threonine residue for
the codon encoding the terminal alanine residue in a second amino
acid sequence:
-A-L-L-M-X -A-P-L-A-
1
where X is either alanine, serine or threonine, and where said
1
second amino acid sequence is located between positions 170 and
210 in the EPSP synthase gene.
2. A method of Claim 1 in which the glyphosate-tolerant
EPSP synthase gene is produced from a plant EPSP synthase gene.
3. A method of Claim 1 in which the glyphosate-tolerant
EPSP synthase gene is produced from a bacterial EPSP synthase
gene.
4. A gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-
phosphoshikimate (EPSP) synthase enzyme which encodes a first
amino acid sequence:
-L-G-N-A-A-T-A-
between positions 80 and 120 in the EPSP synthase gene, and
encodes a second amino acid sequence:
-A-L-L-M-X -A-P-L-T-
1
11
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Last modified: November 3, 2007