Ex parte FRASER - Page 3




            Appeal No. 94-2788                                                                                
            Application 07/912,408                                                                            



                                           DECISION ON APPEAL                                                 
                   This is an appeal under 35 U.S.C. § 134 from the final rejection of claims 1 through       
            28.  Subsequently, claim 26 was canceled leaving claims 1 through 25, 27, and 28 for our          
            consideration.  These are all of the claims pending in the application.                           
                   Claims 1, 7, and 13, the independent claims pending in this application are                
            illustrative of the subject matter on appeal and read as follows:                                 
                   1.  A method for rapidly obtaining a culture for detecting esculin hydrolyzing             
            procaryotes via nucleic acid hybridization techniques wherein the step of plating the culture     
            is eliminated, the improvement comprising pelleting an enrichment culture of the sample           
            and performing a nucleic acid hybridization confirmation test on the sample.                      

                   7.  A rapid method of obtaining a [sic] esculin hydrolyzing procaryotic culture            
            suitable for performing a nucleic acid hybridization confirmation test, comprising:               
                   a) inoculating an enrichment broth with a sample and growing a culture in said             
            enrichment; and                                                                                   
                   b) pelleting said culture.                                                                 

                   13.  A method of rapidly detecting esculin hydrolyzing procaryotes, comprising:            
                   a) inoculating an enrichment broth with a sample and growing a culture in said             
            broth;                                                                                            
                   b) pelleting said culture; and                                                             
                   c) using nucleic acid hybridization techniques to identify said culture.                   



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