Appeal No. 95-1178
Application 07/902,517
acid sequence in a specimen comprising the steps of:
a. combining the specimen with a quantity of a chaotropic agent
sufficient to disrupt molecular structures in cells and to render nucleic
acids available for hybridization, thereby obtaining a mixture;
b. combining the mixture obtained in (a) with a capture probe
consisting essentially of:
(I) a target binding region which is an oligonucleotide
sequence complementary to the target nucleic acid sequence,
and
(ii) a tail region which is a nucleotide sequence that is one
member of a nucleotide affinity pair,
wherein the capture probe is preimmobilized by means of
nucleotide affinity pair binding between the tail region of the
capture probe and a complementary polynucleotide affixed to
a solid polymeric support;
c. maintaining the product of (b) under conditions sufficient for
hybridization of complementary nucleotide sequences to occur,
whereby if the target nucleic acid is present in the specimen, said
target nucleic acid hybridizes to the target binding region of the
preimmobilized capture probe, thereby producing an immobilized
target nucleic acid;
d. labeling the immobilized target nucleic acid with a detectable
agent capable of selectively binding to the target nucleic acid,
whereby a detectably labeled immobilized target nucleic acid is
obtained; and
e. detecting the presence or amount of the detectably labeled
immobilized target nucleic acid.
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Last modified: November 3, 2007