Ex parte HUDSON et al. - Page 4




                 Appeal No. 95-2889                                                                                        Page 4                       
                 Application No. 07/525,231                                                                                                             
                                                                   DISCUSSION                                                                           
                 Appellants request independent consideration of three                                                                                  
                 groups of claims (Paper No. 13 (App. Br.) at 7-9, 22, and                                                                              
                 23) : 3                                                                                                                                
                          Group I:   claims 1-9 and 17-25;                                                                                              
                          Group II:  claims 10-13, 16, 26-28, 29, and 32; and                                                                           
                          Group III: claims 14, 15, 30, and 31                                                                                          
                          The claims                                                                                                                    
                          Claim 1, which is representative of the claimed subject                                                                       
                 matter, is reproduced below:                                                                                                           
                          1.  A method of obtaining at least one obscured                                                                               
                          or partially obscured population analysis from at                                                                             
                          least a portion of a sample having at least a first                                                                           
                          cell population including at least one population                                                                             
                          subset of interest and a second cell population,                                                                              
                          comprising:                                                                                                                   
                                   electronically sensing and counting a first                                                                          
                          population including at least the first cell                                                                                  
                          population and subsets thereof to form a first                                                                                
                          count;                                                                                                                        
                                   shifting the cell population subset of interest                                                                      
                          out of said first cell population and at least                                                                                
                          partially into a second cell population by binding                                                                            
                          microspheres having a reactant bonded thereto                                                                                 
                          specific to said cell population subset of interest                                                                           
                          to said cell population subset;                                                                                               
                                   electronically sensing and counting the                                                                              
                          remaining first population including at least the                                                                             

                          3While Appellants separate the claims into ten groups,                                                                        
                 they state that groups 1, 2, 6, and 7 stand or fall together,                                                                          
                 that groups 3, 4, 8, and 9 stand or fall together, and that                                                                            
                 groups 5 and 10 stand or fall together (Paper No. 13 at 22-                                                                            
                 23).                                                                                                                                   





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