Appeal No. 95-4471
Application No. 07/963,475
1. A diagnostic method comprising the steps of
(i) obtaining a sample of tissue from an organism by biopsy, wherein
said tissue is subject to a fibrosing condition; then
(ii) isolating by microdissection an intact subsegment of said sample,
which subsegment corresponds to a basic organizational structure of said tissue;
(iii) subjecting mRNA from said subsegment to reverse transcription to
obtain cDNA molecules; and thereafter
(iv) bringing said cDNA molecules into contact with PCR primers under
conditions such that a subpopulation of said cDNA molecules undergoes amplification,
wherein cDNAs of said subpopulation encode protein molecules that are involved in
basement membrane-synthetic and -degradative pathways related to said fibrosing
condition; and
(v) analyzing said amplified subpopulation, whereby mRNA levels
corresponding to said amplified subpopulation are monitored and a diagnosis is effected
therefrom.
No references are relied upon by the examiner.
Grounds of Rejection
Claims 1-16 stand rejected under 35 U.S.C. § 112, first paragraph, as being based
on a non-enabling disclosure.
We reverse.
Background
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