Appeal No. 1996-0223
Application No. 07/931,563
(b) contacting said mixture, under conditions under which no metal cation
forms a complex with said protein, with said immobilized antibody to bind said protein
to said immobilized antibody to form an immune complex, and
(c) contacting said immune complex with a metal cation under conditions under
which said metal cation can complex with said protein to induce a stabilized
conformational state of said protein, and thereby release said protein from said
immobilized antibody.
17. The method of claim 2 wherein said protein is capable of forming three
conformational states in the presence of said metal cation, and said antibody is capable
of forming an immune complex with one of said conformational states and is
substantially unreactive with the other two of said conformational states.
The references relied on by the examiner are:
Furie et al. (Furie ‘320) 4,769,320 Sep. 6, 19882
Zimmermann 5,055,557 Oct. 8, 19913
Falb et al. (Falb), “Immobilized Proteins and Peptides” in POLYMERS GRAFTS IN
BIOCHEMISTRY 197-221 (Hixson, Jr. and Goldberg, eds., Marcel Dekker, Inc., New York,
1976).
Furie et al. (Furie 1979), “Conformation-specific Antibodies as Probes of the (-
Carboxyglutamic Acid-rich Region of Bovine Prothrombin,” 254 The Journal of Biological
Chemistry 19, 9766-71 (October 10, 1979).
Swanson et al. (Swanson), “Vitamin K Dependent in Vitro Production of Prothrombin,” 21
Biochemistry 23, 6011-18 (November 9, 1982).
ISSUES
2 Furie ‘320 issued from application no. 661,187, filed October 15, 1984, which was a continuation-in-part of
application no. 402,318, filed July 27, 1982, abandoned, which was a continuation-in-part of application 198,444, filed
October 20, 1980, abandoned.
3 Zimmerman issued from application no. 275,466, filed November 23, 1988, which was a continuation of
application no. 910,315, filed September 2, 1986, which was a continuation of application no. 800,902, filed November
22, 1985, abandoned, which was a continuation of application no. 707,179, filed March 1, 1985, abandoned, which
was a continuation of application no. 472,413, filed March 4, 1983, abandoned.
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