Appeal No. 1996-0848
Application 07/624,053
v) retention of biological activity in the presence of anti-serum to
interferon gamma, interferon alpha, or a combination of anti-sera to
interferon alpha and gamma; and
(c) culturing the clone to produce the continuous cell line.
42. A method of generating a continuous cell line which produces a factor
capable of inducing human monocytes to a cytotoxic state, the method comprising the
steps of:
(a) immortalizing human T-cells to produce continuous cell clones;
(b) identifying a clone which produces a factor having the following
characteristics:
i) capability of inducing human monocytes to a cytotoxic state;
ii) retention of biological activity following treatment at pH 2 for one
hour;
B
iii. retention of biological activity following treatment at 60 C for one
hour;
iv. ability to bind to Matrex Gel Red A under low-salt conditions and
elute from Matrex Gel Red A under high-salt conditions; and
v. retention of biological activity in the presence of anti-serum to
interferon gamma, interferon gamma, interferon alpha, or a
combination of anti-sera to interferon alpha and gamma; and
(c) culturing the clone to produce the continuous cell line.
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