Ex parte KEASLING et al. - Page 2




            Appeal No. 1997-2755                                                                              
            Application 08/311,426                                                                            



                   Claims 1, 3, 8 and 9 are illustrative of the subject matter on appeal and read as          
            follows:                                                                                          
                   1.    A method of bioremediation of organic halide contaminated groundwater,               
            said method comprising the steps of:                                                              
                         (a) adding, in situ to groundwater contaminated with an organic halide and           
            comprising a microbial population capable of reductive dehalogenation of said organic             
            halide, a carbohydrate in an amount sufficient to permit in situ microbial reductive              
            dehalogenation of said organic halide, and thereafter                                             
                         (b) incubating said groundwater in situ under substantially reducing                 
            conditions to completely reductively dehalogenate at least a portion of said organic halide,      
            whereby the concentration of said organic halide in said groundwater is reduced,                  
                         wherein said organic halide is a halogenated hydrocarbon                             
                   3.    A method according to claim 1, wherein said organic halide is                        
            trichloroethene.                                                                                  
                   8.    A method according to claim 1, wherein said carbohydrate is a                        
            monosaccharide or disaccharide.                                                                   

                   9.    A method according to claim 1, wherein said carbohydrate is glucose.                 


                   The reference relied upon by the examiner is:                                              
            Fathepure et al. (Fathepure), “Complete Degradation of Polychlorinated Hydrocarbons by            
            a Two-Stage Biofilm Reactor,” Applied and Environmental Microbiology, Vol. 57, No. 12,            
            (December 1991), pp.3418-3422.                                                                    





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