Appeal No. 1997-4138
Application No. 08/384,681
a. An analytical device consisting essentially of an inert, opaque plastic
carrier having a flat, hydrophobic surface that is non-absorbent to said antigens,
antibodies, haptens and to enzymes, having a multiplicity of visually locatable discrete
locations and, deposited directly on said surface within at least one of said discrete
locations, a spot of an insolubilized specific first binding partner of the at least one
component to be detected;
b. a working reagent conjugate of predetermined concentration of at
least about 15 micrograms per ml in liquid form comprising an enzyme coupled to a
specific second binding partner of the at least one component to be detected; and
c. an enzyme substrate in liquid form comprising a material capable of
reacting with said enzyme at room temperature to form a colored precipitate detectable by
the naked eye against said opaque carrier.
46. A sandwich enzyme-immunoassay process for detecting the presence in a
sample from the group consisting of body fluid and the body fluid diluted 1:10 or less of at
least one component to be detected selected from the group consisting of antigens,
antibodies and haptens, comprising:
a. applying a drop of said sample onto one of a plurality of visually
locatable, discrete locations on a flat, hydrophobic surface, that is non-absorbent to said
antigens, antibodies and haptens and to enzymes, of an inert, opaque plastic carrier, said
location having deposited directly thereon a spot of insolubilized specific, first binding
partner of the at least one component to be detected;
b. incubating said carrier for up to one-half hour to immobilize said at
least one component to be detected;
c. removing unbound body fluid;
d. applying onto each of said discrete locations a drop of a second fluid
comprising at least 15 micrograms per ml of a conjugate of an enzyme coupled to a
specific second binding partner of the component to be detected;
e. incubating at room temperature for up to one-half hour to immobilize
said conjugate;
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