United States Patent (191 pli Patent Number: 4,931,223
Bronstein et al. [451 Date of Patent: Jun. 5, 1990
[541 MEMODS OF USING Zaklika, "Mechanisms... ", J. Amer. Chem. Soc. 100,
CHUMMUMINESCENT 1,2,DIOXL-rANES 4916 (1978).
[751 Inventors: Ireua Y. Bronstein, Newton; John C Schaap, "Chemiluminescence . J. Amer. Chem.
Voyta, North Reading, both of M&U: Soc., 104,3504 (19B2).
[73] Assignee* Tropix, Inc, Bedford, Mam Schaap, "Substituted Effects . . - Tetrahedron Lett.
2943 (1982).
[21] Appl. No.: 213,344 Adam et al., "Thermal Stability... ", Chem. Ber., 116,
[22] Filed: Jun. 30, L988 839(1983).
Pdmmy Examiner-Mary C. Lee
Related U.S. Application Datit Asx&ant Examiner-Catherine Scalzo
[631 Cominuation-in-pint of Ser. No. 989,823, JuL 24,19K Attorney, Agent, or Firm-Lyon & Lyon
and a 0011tinutflim-10-put of Ser. No. 140,035, Dec. 31, (57] AB9rRACr
1987, and a conthmationwin-paft of Ser. No. 140,197,
Dec. 31,1987, abandoned. Processes we disclosed in which fight of different wave
1511 lot CL5 ................CWM 3W/04; C07D 407/04 lengths is simultaneously released from two or more
152] U.S. ............... .......25 00. 435/4; e=ymaticay decomposable chicinfluminescent 1,2
435/5; 435" , dioxetime compouncia, said compounds being config
[581 Field of Seasick .................252/700, 435/4, 5, 7, ured, by in-- of the inclusion of a different light emit
436/537; 549/23, 332, 401, 404, 546/18; ting fluorophore in each of them. to each emit light of
560/117,138; 568/326 said different wavelengilis, by decomposing each of
[561 References Cited laid compounds by meam; of a different enzymm Such
processes can be used in muluýchannel assays-ma.
U.S. PATENT DOCUMENTS Munoamys, chemical. assays and miclac acid probe
3,720,622 3/1973 Bollyky ..........................._ 252/700 aa`nYs--t0 detect the presence or determine the concen
4,663,278 5/1987 DiNollo ............................435/7 tr9fi0n Of ChtlViCal or biological substances, and in
multi-chan-el chemical/physical probe procedures for
OTHER PUBLICATIONS studying the microstractures of macromolecules.
7"llin' "Mechanism Ph-tochem. Photobial. 30,
35(1979). 19 Clams, No Drawings
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