Appeal No. 2001-1675 Page 2
Application No. 08/442,077
(b) preparing a second solution comprising the lipid, the solvent and
an aqueous phase and then removing the solvent so as to form
liposomes, wherein the concentration of the solvent is about 10% by
volume of the solution;
(c) preparing a third solution comprising the lipid, the solvent and an
aqueous phase and then removing the solvent so as to form
liposomes, wherein the concentration of the solvent is from about 10
to 15% by volume of the solution;
(d) determining the mean particle sizes of liposomes prepared in
accordance with steps (a), (b) and (c);
(e) comparing the mean particle sizes with the solvent concentration
used;
(f) selecting an organic solvent concentration corresponding to a
desired mean particle size; and
(g) preparing a solution comprising the lipid, solvent and an aqueous
phase, wherein the organic solvent concentration is selected
according to step (f),
wherein the lipid comprises egg phosphatidylcholine and the solvent
concentration in a solution is at most about 15%.
The reference relied upon by the examiner are:
Teipel 4,039,285 Aug. 2, 1977
Cambiaso et al. (Cambiaso) 4,184,849 Jan. 22, 1980
Cubicciotti et al. (Cubicciotti) 4,619,895 Oct. 28, 1986
Tenzel et al. (Tenzel) 5,000,887 Mar. 19, 1991
Leigh EP 0 158 441 Oct. 16, 1985
GROUND OF REJECTION
Claims 21-26 and 28-29 stand rejected under 35 U.S.C. § 103 as being
unpatentable over Tenzel in view of Leigh with or without Cubicciotti, Teipel or
Cambiaso.
We reverse.
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