Appeal No. 2002-1926 Page 2
Application No. 08/693,353
appeal and read as follows:
23. A process for preparing a composition having bactericidal activity
comprising:
culturing cells of a strain of Micrococcus varians, which upon culturing in a
culture medium, produces a bacteriocin which has agar well inhibition test activity
against bacterial strains including Listeria monocytogenes to obtain cultured cells in a
concentration of from 107 to 1011 organisms per ml of the medium and a supernatant
comprising the bacteriocin; and
separating the supernatant from the cultured cells to obtain the supernatant for
obtaining a supernatant composition comprising the bacteriocin.
24. A process according to claim 23 wherein bacteriocin comprises an amino
acid sequence selected form the group consisting of SEQ ID NO:1 and a sequence
differing from SEQ ID NO:1 by from 1 to 4 amino acids.3
36. The supernatant composition of the process of claim 23 or 24.
42. A cell-free Micrococcus varians culture-medium-supernatant composition
comprising a bacteriocin which has agar well incubation inhibition test activity against
bacterial strains including Listeria monocytogenes.
45. A bacteriocide which comprises a bacteriocin isolated from a bacteria and
purified and which comprises an amino acid sequence selected from the group
consisting of SEQ ID NO:1 and a sequence differing from SEQ ID NO:1 by from 1 to 4
amino acids.
46. A bacterioside according to claim 45 wherein the bacteriocin comprises
amino acid sequence SEQ ID NO:1.
The reference relied on by the examiner is:
Cantoni et al. (Cantoni), “Bacteriocins from Micrococcaceae,” Industrie Alimentarie, Vol.
31, pp. 660-665 (July-August 1992)
DISCUSSION
Claims 23-26, 28, 30, 32, 36, 38, 40, 42-47 and 51-54 stand rejected under the
3 As explained in the specification (pages 3,14 and 20), concentrated culture
supernatants from Micrococcus varians strains CNCM I-1586 and CNCM I-1587 were
found to inhibit various species of bacteria, including Listeria monocytogenes, in agar
well tests. Inhibitory fractions of the culture supernatants were found to contain a
proteinaceous bacteriocin, termed “variacin.” SEQ ID NO:1 represents the amino acid
sequence of variacin.
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