The opinion in support of the decision being entered today was not written
for publication and is not binding precedent of the Board.
Paper No. 28
UNITED STATES PATENT AND TRADEMARK OFFICE
__________
BEFORE THE BOARD OF PATENT APPEALS
AND INTERFERENCES
__________
Ex parte TAKASHI ETOH and MAMORU TAKAHASHI
___________
Appeal No. 2003-1060
Application No. 09/101,6951
__________
ON BRIEF
__________
Before SCHEINER, ADAMS and GREEN, Administrative Patent Judges.
SCHEINER, Administrative Patent Judge.
DECISION ON APPEAL
This is a decision on appeal under 35 U.S.C. § 134 from the final rejection of
claims 2-7 and 15, the only claims remaining in the application.
Claim 15 is representative:
15. A method for assaying ammonia and/or ammonium ions in a liquid sample,
said method comprising:
a) performing a main reaction comprising incubating said sample with NAD
synthetase, ATP, desamido-NAD and at least one of Mg2+ and Mn2+ ions, thereby to
generate oxidized NAD;
b) converting said oxidized NAD to reduced NAD in a secondary reaction
system comprising an amine substrate and a dehydrogenase that catalyzes a reaction
(i) generating ammonia molecules from said amine substrate and (ii) generating said
reduced NAD from said oxidized NAD;
wherein said ammonia molecules generated in step b) then participate in
continuing said main reaction of step a), thereby to effect cycling of ammonia molecules
1 Application for patent filed September 17, 1998.
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Last modified: November 3, 2007