Appeal No. 2003-1594 Page 2
Application No. 08/970,266
(b) culturing the said cells under conditions suitable for glutamine
synthetase selection and recombinant antibody gene expression.
11. A method for expressing a recombinant gene in a murine host cell
comprising the following steps:
a) inserting said recombinant gene into a murine immunoglobulin
gamma 2A locus present in said murine host, wherein said
recombinant gene comprises a nucleic acid encoding for a
recombinant protein and a promoter transcriptionally coupled to
said nucleic acid providing for expression in said murine host,
provided that said recombinant gene is capable of being expressed
is [sic] said murine host, and
b) culturing said host under conditions suitable for expression of said
recombinant gene.
The examiner relies upon the following references:
Fell et al. (Fell A) 5,204,244 Apr. 20, 1993
Hollis et al. (Hollis) WO 95/17516 Jun. 29, 1995
Yuriko Yamawaki-Kataoka et al. (Yamawaki-Kataoka), ”The complete nucleotide
sequence of mouse immunoglobulin K2a gene and evolution of heavy chain
genes: further evidence for intervening sequence-mediated domain transfer,”
Nucleic Acids Research, Vol. 9, No. 6, pp. 1365-1381 (1981)
Galfre et al. (Galfre), ”Preparation of Monoclonal Antibodies: Strategies and
Procedures,” Methods in Enzymology, Vol. 73, pp. 3-48 (1981)
Sambrook, Molecular Cloning A Laboratory Manual, 2nd Edition, pp. 16.8-16.15
(1989)
Fell et al. (Fell B), “Homologous recombination in hybridoma cells: Heavy chain
chimeric antibody produced by gene targeting,” Proc. Natl. Acad. Sci. USA, Vol.
86, pp. 8507-8511(1989)
Claim 13 stands rejected under 35 U.S.C. § 112, first paragraph, as
containing subject matter that was not described in the specification in such a
way as to reasonably convey to one skilled in the relevant art that the inventors,
at the time of filing, had possession of the claimed invention. Claim 13 stands
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