Appeal No. 2004-1734
Application No. 08/942,369
38. A method of detecting the presence of urinary pathogens in a
biological sample and of simultaneously determining the susceptibility of the
urinary pathogens to antimicrobial agents, said method comprising:
providing a multicompartment assay device comprising:
at least one compartment comprising a medium capable of sustaining
growth of total microbial organisms; at least one compartment comprising a
uropathogenic specific medium comprising yeast extract; and, at least one
compartment comprising an antimicrobial susceptibility interpretation medium;
placing a portion of the biological sample respectively in said at least one
compartment comprising a medium capable of sustaining growth of total
microbial organisms; said at least one compartment comprising a uropathogenic
specific medium comprising yeast extract; and, said at least one compartment
comprising an antimicrobial susceptibility interpretation medium comprising an
antimicrobial agent;
whereby metabolism of a signal generating substrate and production of a
detectable signal in said at least one compartment comprising a medium capable
of sustaining growth of total microbial organisms indicates the presence of
microbial organisms in a sample; metabolism of a signal generating substrate
and production of a detectable signal in said at least one compartment
comprising a uropathogenic specific medium comprising yeast extract indicates
the presence of urinary pathogens in the sample; and metabolism of a signal
generating substrate and production of a detectable signal in said at least one
compartment comprising an antimicrobial susceptibility interpretation medium
indicates that the organisms lack susceptibility to the antimicrobial agent
comprised in said antimicrobial susceptibility interpretation medium; and
examining the compartments to determine the presence of urinary
pathogens in said biological sample and the susceptibility of said urinary
pathogens to said antimicrobial agents.
43. The method of claim 38 wherein the at least one antimicrobial susceptibility
interpretation medium comprises amoxicillin, clavulanic acid/amoxicillin, or
enrofloxacin.
3
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Next
Last modified: November 3, 2007