Appeal No. 93-3114
Application 07/825,465
Maas analyzed the results as follows (page 170, last two
lines, through page 171, first full paragraph; emphasis
added):
Leukotoxin neutralizing activity in specific
bovine antiserum to the leukotoxic substances of
F. necrophorum has not been previously reported.
In this study, an increased macrophage viability
was demonstrated when 2 leukotoxin types were
incubated with sera from calves that had been
immunized or infected with FN 3080 preparations
(Tables 3 and 4). This viability was significantly
greater than that observed with serum from calves
that had received only PBS injections.
These data indicate the presence of a specific
leukotoxin neutralizing component in the bovine sera.
The sera . . . from F. necrophorum-immunized and
-infected calves (no. 74, 78 and 76; Table 2) used in
this leukotoxin neutralization assay were positive for
anti-F. necrophorum antibody with the ELISA screen. The
detection of specific antibody and specific leukotoxin
neutralizing activity in the same serum samples from
calves immunized or infected with F. necrophorum but
not in the control serum suggests that specific
antitoxin may be generated in the bovine using
F. necrophorum vaccines and may, therefore, be an
important aspect of protection in vivo.
However, Maas also found that (pages 180-181, bridging
paragraph):
Peripheral blood lymphocytes from F. necrophorum-
immunized and -experimentally infected calves did
not demonstrate uniform specific stimulation when
cultured with a ribosome-rich antigen prepared from
F. necrophorum. However, these calves did produce
anti-F. necrophorum antibody as assessed by the enzyme-
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