Appeal No. 94-4081
Application 07/750,031
preparing a medium for separating a mixture
including charged components wherein the separation is based on
differential interaction;
distributing generally uniformly through the
medium the buffer solution;
causing separation of at least the charged
components of the mixture based on differential interaction
through the medium where like-charged flourophore [sic,
fluorophore] components are displaced by like-charged mixture
components;
laser scanning a laser beam in a two dimension
scan across the medium after separation of components of the
mixture;
detecting variations in fluorescing light
intensity correlated to location of the laser beam during the
scan across the medium; and
determining the presence or absence of mixture
components at certain locations by the level of light intensity
at certain locations, the light intensity or lack thereof
comprising of physical characteristic of the flourophore [sic,
fluorophore] and not of the mixture.
46. The method of claim 37 [sic, claim 37 was
renumbered as present claim 42] wherein the medium is contained
within a capillary tube.
The examiner has relied upon the following reference as
evidence of obviousness:
Ma et al. (Ma), “Indirect Fluorometric Detection of Anions in
Thin-Layer Chromatography”, 60 Anal. Chem., No. 7, 722-724 (April
1, 1986).
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