Appeal No. 95-0635
Application 07/768,255
1. A method for determining the glucose content in whole
blood, in which a sample of undiluted whole blood is contacted
with a dye-containing reagent system which undergoes chemical
reaction with the glucose in the whole blood sample, the method
consisting essentially of the steps of:
introducing the undiluted whole blood sample in a
microcuvette having at least one cavity for receiving the sample,
said cavity being internally pretreated with the reagent in a dry
form and said chemical reaction then taking place in said cavity,
the reagent being comprised of a hemolyzing agent and agents
used in the glucose dehydrogenase method, said agents being
comprised of glucose dehydrogenase and a redox indicator dye, the
hemolyzing agent exposing the glucose contained in the blood
cells of the whole blood sample permitting a quantitative total
glucose determination in a whole blood hemolysate, the agents
which participate in the chemical reaction ensure that a dye
concentration change takes place in a wavelength range above
650 nm and,
performing an absorption measurement at said wavelength
range directly on the sample in the cuvette, and further
conducting a secondary absorption measurement to compensate for
background interference in a wavelength range above 700 nm.
8. A disposable cuvette for carrying out a determination of
the glucose content of undiluted whole blood where a sample of
undiluted whole blood is contacted with a dye-containing reagent
system undergoes chemical reaction with the glucose in the
undiluted whole blood sample, the cuvette being comprised of at
least one cavity for receiving the undiluted whole blood sample,
said cavity being internally pretreated with a reagent in dry
form and said chemical reaction occurring in said cavity after
introduction of the sample in undiluted form, the reagent being
comprised of a hemolyzing agent and agents used in the glucose
dehydrogenase method, said agents being comprised of glucose
dehydrogenase and a redox indicator dye, the hemolyzing agent
exposing the glucose contained in the blood cells of the whole
blood sample permitting a quantitative total glucose
determination in a whole blood hemolysate, the agents which
participate in the chemical reaction ensure that the dye
concentration change takes place in a wavelength range above
650 nm and that the cuvette is at least partly transparent for
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Last modified: November 3, 2007