Appeal No. 1996-2013
Application No. 07/935,695
(ii) transforming a host cell with the genetic sequence;
(iii) expressing the genetic sequence in the host cell; and
(iv) recovery of single chain polypeptides which have non-covalently linked to form
dimers and multimers.
The references relied on by the examiner are:
PCT Application (Huston) WO 88/09344 Dec. 1, 1988
Colcher et al. (Colcher), “In Vivo Tumor Targeting of a Recombinant Single-Chain Antigen-
Binding Protein,” Journal of the National Cancer Institute, Vol. 82, No. 14, pp. 1191-1197
(1990).
Rodwell et al. (Rodwell), “Linker Technology: Antibody-Mediated Delivery Systems,”
Biotechnology, Vol. 3, pp. 889-894 (1985).
Sheer et al. (Sheer), “Purification and Composition of the Human Tumor-Associated
Glycoprotein (TAG-72) Defined by Monoclonal Antibodies CC49 and B72.3,” Cancer
Research, Vol. 48, pp. 6811-6818 (1988).
Pantoliano et al. (Pantoliano), “Conformational Stability, Folding, and Ligand-Binding
Affinity of Single-Chain Fv Immunoglobulin Fragments Expressed in Escherichia coli,”
Biochemistry, Vol. 30, pp. 10117-10125 (1991).
All of the claims stand rejected under 35 U.S.C. § 103. In regard to claims 1, 3, 4, 8
through 18, 21 and 23, the examiner relies on Huston, Colcher, Rodwell and Sheer as
evidence of obviousness. In regard to claims 24 and 25, the examiner relies on Huston,
Sheer and Pantoliano. We reverse.
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