Appeal No. 2001-0562 Page 2
Application No. 08/460,478
Claims 81 and 122 are illustrative of the subject matter on appeal and are
reproduced below:
81. A method for expressing a gene in mammalian central nervous
system cells, which method comprises
modifying said cells to contain a replication-deficient recombinant
adenovirus comprising said gene under the control of a promoter,
wherein said gene encodes a polypeptide molecule functioning in
said cells as a neurotransmitter-synthesizing enzyme, trophic
factor, a growth factor, or a lysosomal enzyme, or wherein said
gene is an antisense sequence, the transcription product of which
blocks the expression in said cells of proteins responsible for
neuropsychiatric disease, or of enzymes involved in the
biosynthesis of said proteins or in the biosynthesis of glutamate;
and
wherein the gene is expressed in said modified cells.
122. A method for treating Parkinson’s Disease which method comprises
sterotactically administering to the brain of a mammal suffering from
Parkinson’s Disease a pharmaceutical composition comprising a
replication-defective adenovirus which infects central nervous
system cells of said mammal and expressing a nucleotide
sequence, contained in said adenovirus, which nucleotide sequence
encodes a neurotransmitter-synthesizing enzyme whereby said
subject is treated for Parkinson’s disease.
The references relied upon by the examiner are:
Geller et al. (‘979) 5,501,979 Mar. 26, 1996
Geller et al. (‘945) WO 92/07945 May 14, 1992
Braithwaite, “Semipermissive replication of adenovirus 5 in rat brain cells and
evidence for an induction of cellular DNA replication in vivo,” J. gen. Virol., Vol.
67, pp. 391-396 (1986)
Stratford-Perricaudet et al. (Stratford-Perricaudet), ”Gene transfer into animals :
the promise of adenovirus,” Human Gene Transfer, Vol. 219, pp. 51-67 (1991)
Ikenaka et al. (Ikenaka), ”Detection of brain-specific gene expression in brain
cells in primary culture: A novel promoter assay based on the use of a retrovirus
vector,” The New Biologist, Vol. 4, No. 1, pp. 53-60 (1992)
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