Appeal No. 2001-0756 Page 2
Application No. 08/666,400
compound in methanol in the presence of triethylamine to form
active matrices with active groups;
b) dissolving a protein containing at least one primary or
secondary amino group and adding the protein to the activated
matrices;
c) incubating the activated matrices and the protein of step b) at a
pH of 7-10 and a temperature of +4ºC to +60ºC in an aqueous
buffer system, free of primary and secondary amines, to thereby
immobilize the protein of step b) on the matrices.
The references relied upon by the examiner are:
Arnold 4,560,504 Dec. 24, 1985
Lau 4,952,519 Aug. 28, 1990
Cuatrecasas et al. (Cuatrecasas), Affinity Chromatography, in Methods in
Enzymology, Vol. XXII, pp. 345-378 (William B. Jakoby ed., Academic Press)
(1971)
(Harlow), ANTIBODIES, A Laboratory Manual, pp. 522-32, and 528 (Ed Harlow
et al., eds., Cold Spring Harbor Laboratory) (1988)
Glüsenkamp et al., “Squaric Acid Diethylester: A Simple and Convenient
Coupling Reagent,” J. Biosciences, Vol. 46, pp. 498-501 (1991)
Tietze et al. (Tietze), “Squaric Acid Diethyl Ester: A New Coupling Reagent for
the Formation of Drug Polymer Conjugates. Synthesis of Squaric Acid Ester
Amides and Diamides,” Chem. Ber., Vol. 124, No. 5, pp 1215-21 (1991)
GROUND OF REJECTION
Claims 58-67 stand rejected under 35 U.S.C. § 103 as being unpatentable
over Tietze or Glüsenkamp in view of Harlow, Arnold, Cuatrecasas and Lau.
We reverse.
DISCUSSION
The examiner relies (Answer, page 5) on Tietze and Glüsenkamp in the
alternative to “teach the use of a cyclobutane carboxylic acid derivative to
conjugate amine bearing molecules conducting the reaction in either inert
solvents (viz, ethanol) or non-amine containing aqueous buffers at pH7.”
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