Appeal No. 2002-1355 Page 8
Application No. 08/907,783
(e.g. monocytes, macrophages) are required. The T2 cells are not functioning as
antigen presenting cells (which is to be expected since the T2 cells lack antigen
presenting Major Histocompatibility Complex antigens HLA-DR and HLA-A,B,C),
but rather function as accessory cells.” Page 14.
In Yokozeki’s assay system, by contrast, the keratinocytes function as
antigen-presenting cells. See Yokozeki, page 394: “We conducted a study on
the primary in vitro activation of T cells from non-sensitized mice by using
hapten-conjugated Pam 212 cells (keratinocyte cell line). . . . Monolayered Pam
212 cells were incubated with a variety of chemicals exhibiting allergic
potential. . . . T cells and macrophages . . . were cocultured for 5 days with those
monolayered Pam cells conjugated with chemicals.” See also page 399:
[W]e examined whether or not primary activation of T cells from
nonsensitized Balb/c mice can be induced by hapten-conjugated
Pam cells and macrophages instead of hapten-conjugated
Langerhans [dendritic] cells. . . .
The role of the Pam cells and macrophages in our system remains
unclear. . . . Fahr et al. recently suggested that keratinocyte (KC)-
derived protein but not 3T3-fibroblast-derived protein can serve as
antigenic carriers for hapten. Our data are consistent with those of
Fahr et al.
One possibility of the mechanism in our system is that
macrophages may play a role as antigen-processing cell and be
required as the source of a costimulatory signal (2nd signal) and
the Pam cell[s] may serve as antigen carriers for hapten. This
possibility is currently under investigation.
(Reference citations omitted.)
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