into their precursor cells in vivo or in vitro. However, materials and methods
of the invention can reprogram differentiated cells into immortalized,
totipotent cells. Differentiated cells can be isolated from a fetus or a live bom
animal, for example.
Strelchenko Application 09/357,445, Paper I (specification), p. 17,11. 17-23.
F 54- According to Strelchenko's written description, the conversion of non-totipotent cells to
totipotent cells prior to their use as donor cells is an important aspect of Strelchenko's
process and allows virtually any cell to be used as a donor cell. Strelchenko Application
09/357,445, Paper I (specification), p. 12,11. 3-11 and p. 17,1. 28 - p. 18,1. 2.
F 55. According to Strelchenko a non-totipotent cell may be converted to a totipotent cell using
"features of the invention:"
a non-totipotent precursor cell can be converted into a totipotent cell by
utilizing features of the invention described hereafter.
Strelchenko Application 09/357,445, Paper I (specification), p. 12,11. 11-12.
F 56. Strelchenko calls this conversion of non-totipotent to totipotent cells "reprogramming:"
This conversion process can be referred to as a reprogramming step.
Strelchenko Application 09/357,445, Paper I (specification), p. 12,1. 13.
F 57. Strelchenko's specification further describes reprogramming:
The term "reprogramming" or "reprogrammed" as used herein refers to
materials and methods that can convert a non-totipotent cell into an totipotent
cell.
Strelchenko Application 09/357,445, Paper I (specification), p. 12,11. 17-18.
F 58. The reprogramming of non-embryonic cells to make them totipotent is said to be a unique
feature of Strelchenko's invention:
A unique feature ofthe present invention isthat immortalized, totipotentcells
are reprogrammed from non-embryonic cells by utilizing the materials and
methods described herein in descriptions of the preferred embodiments and
exemplary embodiments.
Strelchenko Application 09/357,445, Paper I (specification), p. 17,1. 28 - p. 18,1. 2.
F 59. Strelchenko further emphasizes the importance of reprogramming in allowing cloning to be
carried out from virtually any type of "precursor" cell:
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