Appeal 2007-0849
Application 10/106,649
INTRODUCTION
The claims are directed to a method for imparting an anti-microbial
activity or enhancing the anti-microbial activity of an article for personal
care or a functional product. Claim 18 is illustrative:
18. A method for imparting an anti-microbial activity or enhancing
the anti-microbial activity of an article for personal care or a functional
product which comprises adding to said personal care article or to said
functional product an anti-microbial composition containing at least 30% by
weight of one or more active perfuming ingredients, the anti-microbial
activity of which, against at least 2 different kinds of bacteria selected from
the group consisting of Escherichia coli, Pseudomonas aeruginosa,
Staphylococcus aureus and Enterococcus hirae, is l00%, as measured by a
method to evaluate the antimicrobial activity which comprises:
a) solubilizing the ingredient to be tested in an aqueous medium in a
concentration between 250 and 1000 μg/ml, relative to the medium, in the
presence of an effective amount of solvent which is substantially non-toxic
for bacteria and which allows a complete solubilizing of the perfuming
ingredient;
b) adding an inoculum of the desired bacteria such that the final
concentration in the medium is at least l07 colony forming units/ml of the
medium;
c) diluting the medium so as to reduce the bacteria concentration to
103 colony forming units/ml of medium;
d) spreading l02 colony forming units/ml of the bacteria onto an
appropriate culture medium,
e) counting the surviving colonies after incubation; and
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Last modified: September 9, 2013