Appeal 2007-1070
Application 09/467,901
1. A method of detecting and/or quantifying an IgE
antibody specific to a ligand in the form of an antigen, an
antibody, or a hapten in a liquid sample suspected to contain the
IgE antibody comprising the steps of:
(a) contacting (i) the sample with (ii) a free dissolved
ligand in the form of an antigen, an antibody, or a hapten to
form a mixture I comprising complexes that comprise the IgE
antibody and the ligand (IgE-containing complexes),
(b) mixing the mixture I with a carrier to which is
bound (iii) an IgE receptor, wherein said IgE receptor is CD23
(FсεRII) and/or FсεRI, to form a mixture II comprising carrier-
bound IgE-containing complexes,
(c) separating the carrier-bound IgE-containing
complexes from the mixture II, and
(d) determining the amount of the carrier-bound IgE-
containing complexes formed by detecting a label present in the
carrier-bound IgE-containing complexes,
wherein the label to be detected is associated with the
ligand or the IgE antibody and wherein the label to be detected
is added to the complexes present in steps (a), (b), or (c) and
does not form part of the carrier.
6. A method of detecting and/or quantifying an IgE
antibody specific to a ligand in the form of an antigen, an
antibody, or a hapten in a liquid sample suspected to contain the
IgE antibody comprising the steps of:
(a) contacting (i) the sample with (ii) a free dissolved
ligand in the form of an antigen, an antibody, or a hapten to
form a mixture I comprising complexes that comprise the IgE
antibody and the ligand (IgE-containing complexes),
3
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