Appeal No. 93-2460
Application No. 07/590,647
encode the Pseudomonas aroA gene. Fitzgibbon, pp. 32 and 96.
Thus, Fitzgibbon concludes that expression of a resistant EPSPS
gene may not be the only way to achieve glyphosate resistance.
Id. at p. 28, last para.
DeGreve discloses the use of genetic engineering techniques
to transform plant cells and their progeny to express the Bt2
toxin derived from Bacillus thuringiensis. According to DeGreve
the successful transformation and expression of the Bt2 toxin may
be more difficult than other genes for one or more reasons such
as:
(1) the large size of the Bt2 toxin, even in its
truncated form; (2) the particular properties of
the Bt2 polypeptide (such as, but not limited to,
solubility of the polypeptide); (3) the potential
toxicity of the Bt2 polypeptide toward the plant
cells; or (4) the Bt2 polypeptide synthesized in
plant cells and their progeny must retain
substantially the same properties as the crystal
protein synthesized in bacteria.
DeGreve, para. bridging pp. 3-4.
Potrykus provides a brief review of methods to produce
transgenic cereals. According to Potrykus, it has not been
generally possible to transform cereals (which are
monocotyledonous plants) using Agrobacterium as the vector.4
4Potrykus notes that standard direct gene transfer
(continued...)
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