Appeal No. 1996-0449
Application 08/009,423
83. Method for increasing production of a protein which contains arginine,
comprising:
(i) transforming an Escherichia coli host cell with an expression vector which
contains a DNA sequence, wherein said DNA sequence (a) codes for said arginine
containing protein, and (b) contains codons AGG and AGA;
(ii) transforming said Escherichia coli host cell with an extrachromosomal
expression vector which contains a DNA sequence which codes for a tRNA, wherein said
tRNA (a) incorporates arginine into protein, (b) recognizes both of codons AGA and AGG,
and (c) contains anticodon UCU, and
(iii) treating said transformed host cells under conditions favoring an increase in
expression of said DNA sequence which codes for said tRNA, so as to produce said
arginine containing protein in an amount greater than the amount of said arginine
containing protein produced by said host cell prior to transformation.
The references relied upon by the examiner are:
George et al. (George) 4,673,641 June 16, 1987
Huala et al. (Huala) 4,970,147 Nov. 13, 1990
Tabor et al. (Tabor) 5,145,776 Sept. 8, 1992
Neill et al. (Neill), "Expression of a Wheat "-Gliadin Gene in Saccharomyces cerevisiae,"
Gene, Vol. 55, pp. 303-17 (1987).
Miller et al. (Miller), "Protein Modification," Journal of Cellular Biochemistry, Supplement
11C, page 195 (1987).
Garcia et al. (Garcia), "The E. coli dnaY Gene Encodes an Arginine Transfer RNA," Cell,
Vol. 45, pp. 453-59 (1986).
Robinson et al. (Robinson), "Codon Usage Can Affect Efficiency of Translation of Genes in
Escherichia coli," Nucleic Acids Research, Vol. 12 (17), pp. 6663-671 (1984).
Bonekamp et al. (Bonekamp), "The AGG Codon is Translated Slowly in E.coli
Even at Very Low Expression Levels," Nucleic Acids Research, Vol. 16 (7), pp.
3013-024(1988).
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