Appeal No. 1998-1382
Application No. 08/330,717
coextruding the polystyrene and the polymer
alloy blend producing a layered film having a gas
permeability to promote cell growth.
36. A flexible, gas-permeable cell culture
container suitable for culturing cells, the
container comprising:
a first side wall of the container being
suitable for growing adherent cells, the first side
wall comprises a first layer of polystyrene having a
thickness within the range of 0.0001 inches to about
0.0010 inches, and, a second layer adhered to the
first layer of a polymer alloy having multiple
components, the second layer having a thickness
within the range of 0.004 inches to about 0.015
inches;
a second side wall attached to the first side
wall for growing non-adherent cells; and,
means associated with the container for
distinguishing the first side wall from the second
side wall.
38. A method for culturing cells comprising the
steps of:
providing a flexible cell culture container
having at least one side wall of a film having a
first layer of a polystyrene having a thickness from
0.0001 inches to about 0.0010 inches, and a second
layer adhered to the first layer of a polymeric
material having a thickness from 0.004 inches to
about 0.015 inches, the first layer faces an
interior of the container to provide a cell growth
surface;
adding to the container a cell growth medium;
and
seeding the container with cells to be grown.
The subject matter on appeal relates to (i) a multi-
layer, flexible, gas-permeable film suitable for forming a
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