Appeal No. 1998-1382
Application No. 08/330,717
cell culture container (claims 1 and 21), (ii) a flexible,
gas-permeable cell culture container (claims 32 and 36), (iii)
a method for fabricating a multi-layered film suitable for
forming a container for culturing cells (claim 34), and (iv) a
method for culturing cells (claim 38). The film comprises a
first layer composed of a polystyrene and a second outer layer
adhered to the first layer and composed of a polymer alloy
blend (claims 1, 21, 34, and 36), a polyolefin (claim 32) or a
polymeric material (claim 38), with the first and second outer
layers having the recited thicknesses. According to the
appellants, the present invention provides a layer of
polystyrene as part of a layered film without rendering the
film too stiff to fabricate a flexible container and too
impermeable to allow the passage of certain gases necessary to
sustain cell growth in an unvented, flexible container.
(Appeal brief, pages 4-5.)
The examiner relies upon the following prior art
references as evidence of unpatentability:
Erb 3,589,976 Jun. 29,
1971
Stanley et al. 3,655,503 Apr.
11, 1972
5
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