Appeal No. 1999-1423
Application 08/261,639
Claim 48 is illustrative of the claims on appeal and read as follows:
48. A device for detecting the presence or amount of HIV-1 or HIV-2 antibody
in a biological sample, comprising:
a sample absorber pad for application of said sample;
a first matrix, said first matrix being in fluid communication with said sample
absorber pad, said first matrix being impregnated with a first glycoprotein covalently
linked to bovine serum albumin, said first glycoprotein being selected from the group
consisting of HIV-1 glycoprotein 41 and HIV-2 glycoprotein 36, said glycoprotein
covalently linked to bovine serum albumin being conjugated to gold particles having a
diameter of between about 5 nm and about 50 nm; and
a second matrix in fluid communication with the first matrix, said second matrix
having immobilized thereon a point spatially separated from the first matrix a second
glycoprotein covalently linked to bovine serum albumin, said second glycoprotein being
HIV-1 glycoprotein 41 when said first glycoprotein is HIV-1 glycoprotein 41 and being
HIV-2 glycoprotein 36 when said first glycoprotein is HIV-2 glycoprotein 36, said second
glycoprotein being immobilized on the second matrix;
wherein said first glycoprotein is capable of moving from the first matrix to the
second matrix and to said point spatially separated from the first matrix after application
of the sample to the first zone.
The prior art references relied upon by the examiner are:
Kondo et al. (Kondo) 4,256,693 Mar. 17, 1981
Weng et al. (Weng) 4,740,468 Apr. 26, 1988
Tzeng et al (Tzeng) WO 92/22797 Dec. 23, 1992
Berry et al. (Berry), “A comparison of four enzyme immunoassays for the simultaneous
detection of HIV-1- and HIV-2-specific antibody, Journal of Virological Methods, Vol. 34,
pp. 91-100 (1991)
2
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 Next
Last modified: November 3, 2007