Appeal No. 2006-0102 Page 2
Application No. 09/732,439
61. A fertile transgenic Zea mays plant comprising a recombinant DNA
segment comprising a promoter operably linked to a first DNA
segment encoding an enzyme which catalyzes the synthesis of the
osmoprotectant proline, wherein the first DNA segment is expressed
so that the level of the enzyme is increased in transgenic Zea mays
plant, and wherein the recombinant DNA segment is heritable.
The references relied upon by the examiner are:
Verma et al. (Verma I) 5,344,923 Sep. 6, 1994
Verma et al. (Verma II) 5,639,950 Jun. 17,1997
Adams et al. (Adams ’98) 5,780,709 Jul. 14, 1998
Adams et al. (Adams ’01) 6,281,411 Aug. 28, 2001
Barnett et al. (Barnett), “Amino acid and protein metabolism in Bermuda grass
during water stress,” Plant Physiol., Vol. 41, pages 1222-1230 (1966)
Jones et al. (Jones), Physiology and Biochemistry of Drought Resistance in
Plants, Ch. 9, Betaines, pp. 171-204 (Academic Press, Australia) (1981)
Rayapati et al. (Rayapati), “Pyrroline-5-Carboxylate Reductase Is in Pea (Pisum
sativum L.) Leaf Chloroplasts,” Plant Physiol., Vol. 91, pp. 581-586 (1989)
McCue et al. (McCue), “Drought and salt tolerance: towards understanding and
application,” TIBTECH, Vol. 8, pages 358-362 (1990).
Brandriss et al. (Brandriss), “Proline biosynthesis in Saccharomyces cerevisiae:
analysis of the PRO3 gene, which encodes Δ1-pyrroline-5-Carboxylate
reductase,” J. Bact., Vol. 174, No. 15, page 5 176 (1992)
Dougherty et al. (Dougherty), “Cloning human pyrroline-5-carboxylate reductase
cDNA by complementation in Saccharomyces cerevisiae,” J. Biol. Chem., Vol.
267, No. 2, pages 871-875 (1992)
Hu et al. (Hu), “A bifunctional enzyme (Δ1-pyrroline-5-carboxylate synthetase)
catalyzes the first two steps in proline biosynthesis in plants,” Proc. Natl. Acad.
Sci., USA, Vol. 89, pages 9354-9358 (1992)
Van Rensburg et al. (Van Rensburg), “Proline accumulation as drought tolerance
selection criterion: its relationship to membrane integrity and chloroplast
ultrastructure in Nicotiana tabacum L.,” J. Plant Physiol., Vol. 141, page 188-194
(1993)
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