Appeal 2007-1624
Application 10/424,662
20. “Aliquot[]s of DPs from each well are mixed together and spread in the
monolayer of required density. This is followed by the necessary number of
fixations” (Drmanac, at col. 7, ll. 27-30).
21. “In this way one can obtain hybridization areas (HA) similar to filters in
dot blot procedure” (Drmanac, at col. 7, ll. 30-31; see also Drmanac, at col.
9, ll. 12-16).
22. “Every HA can in principle by hybridized and reused as classic filters”
(Drmanac, at col. 7, ll. 37-39).
23. The DPs can be labeled with physical attributes or with unique
combinations of oligonucleotides so that they can be recognized and
distinguished from each other (Drmanac, at col. 7, l. 45 to col. 8, l. 21; at
col. 13, ll. 40-45).
24. The exact position of each DP in the hybridization area, and the
corresponding oligonucleotide probe (“ONP”) attached to the DP, can be
established (Drmanac, at col. 9, ll. 19-31 (“By the hybridization of OHAs
[oligohybridization areas] with ONPs . . . , exact position of each ONP in
each OHA is established. OHAs prepared in this way with information on
the position of each ONP ‘product’ . . . can be used for . . . sequence
determination.”)
25. The hybridization areas can be hybridized with different targets and
visualized all at once using a CCD camera having a high density of pixels
(Drmanac, at col. 19, l. 30 to col. 20, l. 18).
26. During processing of the HA (e.g., hybridization and washing) in
detecting genomic DNA, the HA is in contact with a solution (e.g.,
Drmanac, at col. 22, ll. 52-58).
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