Appeal No. 94-1942
Application 07/773,603
11. A process for producing tubers from a tuber-forming
plant belonging to the genus Solanum comprising the steps of:
(a) culturing a plant piece having at least one bud in a
culture vessel containing a liquid medium comprising:
i) a carbon source and
ii) inorganic salts;
(b) exposing said plant piece of step (a) to alternating
periods of light and dark while the plant piece contacts the
medium;
(c) exposing said plant piece to carbon dioxide during at
least one light period of step (b) to induce and propagate
shoots and stolons from said plant piece;
(d) culturing said stolons of step (c) in a liquid medium
containing a carbon source; and
(e) exposing at least a portion of said stolons to a
gaseous phase during step (d) under conditions sufficient to
induce the formation of tubers on said stolons.
The references relied on by the examiner are:
Takayama et al. (Takayama) 5,034,327 July 23, 1991
Angel M. Mingo-Castel et al. (Mingo-Castel), "Effect of Carbon
Dioxide and Ethylene on Tuberization of Isolated Potato Stolons
Cultured In Vitro," 53 Plant Physiology 798-801 (1974).
D. R. Paterson, "Effect of CO Enriched Internal Atmosphere on
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Tuberization and Growth of the Potato," 100 Journal of American
Society of Horticulture Science 431-34 (1975).
R. N. Arteca et al. (Arteca), "Changes in Carbon Fixation,
Tuberization, and Growth Induced by CO Applications to the Root
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Zone of Potato Plants," 205 Science 1279-80 (Sept. 1979).
Motomu Akita et al. (Akita), Mass Propagation of Potato Tubers
Using Jar Fermentor Techniqes," [sic] 230 Acta Horticulturae
55-61 (1988).
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