Appeal No. 94-1695
Application 07/742,088
DECISION ON APPEAL
This is an appeal under 35 U.S.C. § 134 from the final rejection of claims 2, 5, 7
through 10, and 12 through 22, all the claims in the application.
Claims 14 and 22 are illustrative of the subject matter on appeal and read as
follows:
14. A method of amplifying a mixture of different-sequence double-stranded DNA
fragments, comprising
treating the DNA fragments with terminal deoxynucleotide transferase and a
selected deoxynucleotide triphosphate, to add a homopolymeric sequence to the 3N ends
of both DNA fragment strands,
mixing the DNA fragments containing the homopolymeric sequence with (I) a
homopolymer primer that is complementary to said homopolymeric sequence, (ii) heat-
stable DNA polymerase and (iii) all four deoxynucleotide triphosphates,
heat denaturing the DNA fragment strands,
annealing the mixture under conditions to form DNA fragment/primer duplexes,
reacting the mixture under conditions in which the DNA fragment/primer duplexes
are converted to double-stranded DNA molecules,
repeating said denaturing, annealing, and reacting steps until a desired degree of
fragment amplification has been achieved.
22. A method of preparing a mixture of single-stranded RNA fragments for cloning
in a cloning vector, comprising
transcribing the RNA fragments to an RNA/DNA complex using an RNA-strand
primer, where said RNA-strand primer (I) has a sequence complementary to a 3N
2
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Last modified: November 3, 2007