Appeal No. 94-1709
Application 07/716,115
"susceptibility to disease is a primary limiting factor in
potato production internationally" (Shepard, page 189).
Shepard further states that "[w]hen attempting to 'engineer' a
potato possessing specified characteristics, the plant breeder
faces enormous problems only superficially addressed above"
(Shepard, page 191). In a section entitled "Previous Potato
Regeneration from Single Cells or Protoplasts," Shepard
discloses that "[o]nly very recently have techniques emerged
whereby plants may be regenerated from single cells of potato,
whether of mesophyll protoplast or cultured cell origin"
(Shepard, page 192). After describing in detail current
methods used in the laboratory for protoplast isolation and
regeneration, Shepard states as follows:
The foregoing discussion suggests that frequent
examples of potentially valuable variation exist
within potato plant populations raised from
mesophyll protoplasts. Consequently, it is
reasonable to expect that some variants could be
selected early as either protoplasts or small calli
and then be regenerated into plants possessing a
predicted modification. At the present time, there
are no published accounts of this for potatoes, but,
from systems becoming available, ones should be
forthcoming. [Shepard, page 211, emphasis added.]
Finally, in the section entitled "Conclusions," Shepard states
that:
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