Appeal No. 94-2279
Application 07/687,819
1. A composition of matter comprising at least one polypeptide of interest fused to
at least one biotinylated avidin binding polypeptide, wherein the polypeptide of interest and
the biotinylated avidin binding polypeptide each have a N-terminus and a C- terminus and
the fusion takes place at the C-terminus of the biotinylated avidin binding polypeptide.
The references relied upon by the examiner are:
Berger, “Antibodies that Bind Biotin and Inhibit Biotin-Containing Enzymes,” Methods in
Enzymology, vol. 62, pp. 319-326 (1979).
Nagai et al. (Nagai), “Generation of $-Globin by Sequence-Specific Proteolysis of a Hybrid
Protein Produced in Escherichia Coli,” Nature, vol. 309, pp. 810-812 (1984).
Kohanski et al. (Kohanski), “Receptor Affinity Chromatography,” Annals New York
Academy of Sciences, vol. 447, pp. 373-385 (1985).
Lowenadler et al. (Lowenadler), “Production of Specific Antibodies Against Protein A
Fusion Proteins,” The EMBO Journal, vol. 5, pp. 2393-2398 (1986).
Murtif et al. (Murtif), “Mutagenesis Affecting the Carboxyl Terminus of the Biotinyl Subunit of
Transcarboxylase,” The Journal of Biological Chemistry, vol. 262, pp. 11813-11816
(1987).
Maina et al. (Maina), “An Escherichia Coli Vector to Express and Purify Foreign Proteins
by Fusion to and Separation from Maltose-Binding Protein,” Gene, vol. 74, pp. 365-373
(1988).
Sambrook et al. (Sambrook), “Molecular Cloning: A Laboratory Manual,” Cold Spring
Harbor, NY (1989).
Cronan, “Biotination of Proteins in Vivo,” The Journal of Biological Chemistry, vol. 265, pp.
10327-10332 (1990).
Claims 1 through 15 stand rejected under 35 U.S.C. § 103 as unpatentable over
Cronan in view of Maina, Nagai and Lowenadler and further in view of Murtif. Claims 1
3
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