Appeal No. 94-3867
Application 07/808,075
54. A purified composition of matter consisting of a covalently linked compound in
physical admixture with at least one lipid, wherein said covalently linked compound
consists of two parts and has the structural formula: FA-SPC, wherein -SP-C is protein
selected from the group comprising human, porcine, canine and bovine surfactant
associated protein C (SP-C) produced by chemical or enzymatic synthesis or recombinant
DNA means, wherein said SP-C protein comprises a sequence of thirty-four (34) amino
acids, said sequence containing eleven (11) valine amino acid residues, nine (9) of said
valine residues being contained in two (2) adjacent polyvaline stretches, said polyvaline
stretches having a first stretch of six adjacent valine amino acid residues and a second
stretch of three adjacent valine amino acid residues separated from the stretch by two
hydrophobic amino acid residues, said SP-C protein enhances surfactant-like activity of
phospholipids in lungs of an animal, and is substantially resistant to protease,
endoglycosidase F and collagenase enzymes, and wherein FA- is a single fatty acid
selected from the group comprising fatty acids having a carbon chain length from two (2) to
about twenty (20) carbon atoms and wherein said fatty acid is covalently attached to the
amino end terminal amino acid residue of said SP-C.
60. A method for treating hyaline membrane disease or other syndromes
associated with insufficient or abnormal surfactant material said method comprising
administration of an effective amount of a surfactant composition to a patient in need of
treatment, said surfactant composition consisting of at least FS-SPC and at least one lipid
according to claim 54.
62. A purified composition of matter consisting of a covalently linked compound in
physical admixture with at least one lipid, wherein said covalently linked compound
consists of two parts and has the structural formula: FA-SPC, wherein -SP-C is protein
selected from the group comprising human, porcine, canine and bovine surfactant
associated protein C (SP-C) produced by chemical or enzymatic synthesis or recombinant
DNA means, wherein said SP-C protein comprises a sequence of thirty-four (34) amino
acids, said sequence containing eleven (11) valine amino acid residues, nine (9) of said
valine residues being contained in two (2) adjacent polyvaline stretches, said polyvaline
stretches having a first stretch of six adjacent valine amino acid residues and a second
stretch of three adjacent valine amino acid residues separated from the first stretch by two
hydrophobic amino acid residues, said SP-C protein enhances surfactant-like activity of
phospholipids in lungs of an animal, and is
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