Appeal No. 95-4520
Application 08/038,761
1. A compound toxic to European corn borer and having the amino acid sequence
of Bauhinia purpurea lectin except that one or more of the lysine resides is replaced by an
amino acid selected from Ala, Arg, Asn, Gln, Gly, His, Lle, Leu, Met, Phe, Ser, Thr, Trp, Tyr,
and Val.
4. A method according to Claim 3 wherein the diet of the larvae comprises the
tissues of a living plant.
8. A DNA sequence which codes for a compound according to
Claim 1.
9. An expression cassette comprising a DNA sequence according
to Claim 8 operably linked to plant regulatory sequence which cause the expression of the
DNA clone in plant cells.
12. Transformed plant cells containing as foreign DNA at least one
copy of the DNA sequence of an expression cassette according to Claim 9.
18. A larvicidal composition, comprising a European corn borer larvicidal amount
of a compound according to Claim 1 or a combination of
such compounds in a non phytotoxic vehicle.
19. A method of killing or controlling European corn borer larvae in harvested plant
material, comprising applying to the harvested material a composition according to claim
18.
20. A method of killing or controlling European corn borer larvae
in harvested plant material, comprising incorporating into the harvested materials a
compound according to Claim 1 or a combination thereof.
The references relied upon by the examiner are:
Kunkel, Thomas A., “Rapid and efficient site-specific mutagenesis without phenotypic
selection,” 82 Proc. Natl. Acad. Sci, USA, 488-492 (January 1985).
Pratt, Richard C., et al. (Pratt), “Isolation and Partial Characterization of a Seed Lectin
from Tepany Bean that Delays Bruchid Beetle Development,” 93 Plant Physiol.,
1453-1459 (April 1990).
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