Appeal No. 95-1293
Application 07/727,387
Claim 15, which is illustrative of the subject matter on
appeal, reads as follows:
15. A method for the determination of soluble fibrin in
a body fluid, comprising the steps of:
a) binding fibrinogen to a solid phase support;
b) incubating a sample of said body fluid with
the solid phase bound fibrinogen whereby the
soluble fibrin specifically binds to the solid
phase bound fibrinogen, immobilizing the soluble
fibrin;
c) separating said immobilized soluble fibrin
from said body fluid;
d) providing an antibody able to react
immunochemically with the immobilized soluble
fibrin, said antibody being covalently linked to
a detectable label to form a labeled antibody
capable of immunochemically reacting with the
immobilized soluble fibrin;
e) contacting the labeled antibody with the
solid phase bound fibrinogen which has been pre-
incubated with said body fluid to specifically
bind the label to said immobilized soluble
fibrin;
f) detecting said bound label; and
g) determining the concentration of soluble
fibrin in the body fluid from said bound label
wherein said antibody covalently linked to said
detectable label is derived from an animal of
the same species from which the fibrinogen is
obtained but which is not the same species as
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