Appeal No. 95-2889 Page 8
Application No. 07/525,231
Monocytes
(Red/Green)
T- and B-cells
(unlabeled)
2) To obtain a second count, monocytes are again bound to
the same red and green labeled antibodies as in step 1.
In addition, T-cells are bound to antibodies labeled with
the red fluorochrome and B-cells are bound to antibodies
labeled with the green fluorochrome. Some of the
lymphocytes bind to both T- and B-cell antibodies and
thus will be detected as monocytes. A schematic
illustration of the sample as it is labeled for the
second count appears below:
Monocytes
(Red/Green)
T-cells B-cells
(Red) (Green)
3) The number of lymphocytes labeled with both T- and
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Last modified: November 3, 2007