Appeal No. 1996-1038
Application 07/922,492
Claims 7, 8 and 10 are illustrative of the subject matter on appeal and read as
follows:
7. A method of distinguishing between serotypes and/or detecting variants of
infectious bronchitis virus comprising:
a. amplifying the S1 gene region of infectious bronchitis virus;
b. digesting the amplified S1 gene region with HaeIII, or a restriction endonuclease
which cleaves at the HaeIII restriction site, to form a first set of restriction fragments;
c. digesting the amplified S1 gene region with XcmI, or a restriction endonuclease
which cleaves at the XcmI restriction site, to form a second set of restriction fragments;
d. digesting the amplified S1 gene region with BstYI, or a restriction endonuclease
which cleaves at the BstYI restriction site, to form a third set of restriction fragments;
e. separating the restriction fragments within the first, second and third sets by
electrophoresis; and
f. analyzing restriction fragment length polymorphisms to distinguish between
serotypes or detect variants of infectious bronchitis virus.
8. A primer for amplifying the S1 gene region of infectious bronchitis virus
comprising a nucleic acid, having at least 20 nucleotides, which selectively hybridizes
between nucleotide positions -100 through +1, relative to the ATG start site of S1, of the
negative strand of infectious bronchitis virus.
10. A primer for amplifying the S1 gene region of infectious bronchitis virus
comprising a nucleic acid, having at least 20 nucleotides, which selectively hybridizes
between nucleotide positions 1600 through 1700, relative to the ATG start site of S1, of the
positive strand of infectious bronchitis virus.
The references relied upon by the examiner are:
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