Appeal No. 2000-1779 Application No. 08/473,204 homologous to rat GluR1, the second clone was found to be homologous to GluR2. Sun, page 1444, bridging paragraph, columns 1-2. Sun localizes the HBGR2- encoding gene on chromosome 4q25-34.3. Sun does not disclose any specific sequence for GluR2. At this point we find that under high stringency hybridization conditions, a probe for GluR1 cross-reacts with GluR2. Little more is provided in Sun, other than the “note” at page 1447 which states “[a]fter submission of this manuscript a paper [referring to Puckett] appeared reporting … GluH1. This cDNA shows differences with HBGR1 … in a region corresponding to the alternatively spliced exon identified in the rodent clones by Sommer [‘92] … and designated as flip and flop forms of GluR1.” So not only is there cross-reactivity between the receptors, there is also the possibility of alternative splicing events. Puckett, relied upon by the examiner (Answer, page 6) to teach isolation of human GluR1, teaches the use of a reduced stringency hybridization (bridging paragraph pages 7557-558). Furthermore, Puckett also teaches the existence of alternative splicing events (page 7560, column 1), later confirmed by Sun’s “note,” supra. The examiner relies upon Heinemann to teach GluR2 (Answer, page 4). We note Heinemann’s Example 8 (page 27) which teaches “cDNA clones encoding the GluR2 and GluR3 genes were isolated from an adult rat forebrain library using a low-stringency hybridization screening protocol … and a radiolabeled fragment of the GluR1 cDNA as [a] probe.” 57Page: Previous 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 NextLast modified: November 3, 2007