Appeal No. 2000-1780 Application No. 08/403,663 4q25-34.3. At this point we find that under high stringency hybridization conditions, a probe for GluR1 cross-reacts with GluR2. Little more is provided in Sun, other than the “note” at page 1447 which states “[a]fter submission of this manuscript a paper [referring to Puckett] appeared reporting …GluH1. This cDNA shows differences with HBGR1 … in a region corresponding to the alternatively spliced exon identified in the rodent clones by Sommer [‘92]… and designated as flip and flop forms of GluR1.” So not only is there cross-reactivity between the receptors, there is also the possibility of alternative splicing events. Puckett, relied upon by the examiner (Answer, page 5) to teach isolation of human GluR1, teaches the use of a reduced stringency hybridization (bridging paragraph pages 7557-558). Furthermore, Puckett also teaches the existence of alternative splicing events (page 7560, column 1), later confirmed by Sun’s “note,” supra. The examiner relies upon Heinemann to teach GluR2 (Answer, page 4). We note Heinemann’s Example 8 (page 27) which teaches “cDNA clones encoding the GluR2 and GluR3 genes were isolated from an adult rat forebrain library using a low-stringency hybridization screening protocol … and a radiolabeled fragment of the GluR1 cDNA as a probe.” Thus a GluR1 probe cross-reacts with GluR2 and GluR3. Heinemann further teaches that a GluR2 probe cross-reacts with GluR4 and GluR5 (Heinemann, Example 14, page 33). 65Page: Previous 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 NextLast modified: November 3, 2007