Interference No. 103,625
1 2
determined; but it was stated in the ‘072 application that probably X is Pro or Ala, X is
3 4 5 7 6 8 9 10
Ala, X is Ala or Pro, X is Thr X is Thr and X is Val or Leu, with X and X , X and X
identified only as amino acids.
In addition, the ‘072 application specifically discloses the following three
specific N-terminus amino acid sequences (1-3) (Bates 0083)
1 2 3 4 5 6
Sequence 1: FTX YX X EX GSX X RLR
7 8 9 10 1 2 3 4 5 6
Sequence 2: X X FQW X FTX YX X EX GSX X RLR
1 2 3 4 5 6
Sequence 3: TX TX X EX GSX X RLR
The TNF"-binding protein in ‘072 was said to be isolated from the urine of
o
patients with fever ($38 C) by first concentrating the patients urine by ultrafiltration or
reverse osmosis, and then purifying the retentate by ion exchange chromatography
(Example 2, P000079).
The ‘089 application discloses proteins having a molecular weight of about
42,000 daltons and having at the N terminus the amino acid sequences
XTPYAPEPGSTCR which translates to XThrProTyrAlaProGluProGlySerThrCysArg
where X is hydrogen, a phenylalanine residue (F) or the amino acid sequences AF, VAF,
QVAF, AQVAF, PAQVAF or LPAQVAF, and the muteins thereof.
The ‘089 application specifically describes a number of proteins that have
the following N-terminus amino acid sequences:
H-ThrProTyrAlaProGluProGlySerThrCysArg (sequence 3a)
12
Page: Previous 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Next
Last modified: November 3, 2007