Appeal No. 1996-0223
Application No. 07/931,563
through 31, the only other claims pending in the application, have been withdrawn from further
consideration under 37 CFR § 1.142(b) as not readable on the elected invention. 1
Claims 2, 10 and 17 are representative of the subject matter on appeal and read as follows:
2. A method for isolating a vitamin K-dependent protein, which is complexed
with a metal cation and exists in a stabilized conformational state, from a mixture
containing said protein, said method comprising
(a) providing an antibody immobilized on a solid support, said antibody being
reactive with said protein complexed with a divalent or trivalent metal cation and
substantially unreactive with said protein not complexed with said metal cation,
(b) contacting said mixture, in the presence of said metal cation, with said
immobilized antibody to bind said protein, complexed with said metal cation, to said
immobilized antibody to form an immune complex, and
(c) contacting said immune complex with a compound having a binding affinity
for said metal cation higher than the binding affinity of said protein for said metal cation,
to remove said metal cation from said protein to induce a non-stabilized conformational
state of said protein and thereby release said protein from said immobilized antibody.
10. A method for isolating a vitamin K-dependent protein, which is
uncomplexed with any metal cations and exists in a non-stabilized conformational state,
from a mixture containing said protein, said method comprising
(a) providing a conformation-specific antibody immobilized on a solid support,
said antibody being reactive with said uncomplexed protein in a non-stabilized
conformational state and substantially unreactive with said protein complexed with a
metal cation in a stabilized conformational state,
1 Appellants have requested official cancellation of non-elected claims 1, 18-22 and 29-31 in the
amendment filed November 14, 1994 (Paper No. 27), which was not acknowledged by the examiner in the
communication mailed February 23, 1995 (Paper No. 28). This oversight should be corrected upon return of the
above identified application to the jurisdiction of the examiner.
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