Appeal No. 1996-1811
Application No. 08/169,968
APPENDIX
1. A reagent preparation for the rapid production of serum when said
preparation is mixed with a given volume of blood, wherein said mixture
includes thrombin in a concentration range of about 0.2-3.0 units per ml,
snake venom having a concentration in the range of about 0.005-0.2 mg per
ml, and a heparin neutralizing substance including a protamine salt having a
concentration in the range of about 0.02-0.08 mg per ml, said preparation
causing said blood to rapidly clot to enable one to obtain a serum providing
clinically accurate blood chemistry analysis.
14. A cocktail preparation to enable relatively rapid clotting of highly
heparinized blood when said cocktail is mixed with a given volume of blood,
comprising thrombin, a heparin neutralizing substance, and a snake venom
which snake venom is capable of converting fibrinogen to fibrin and is
unaffected by the presence of heparin, said thrombin, heparin neutralizing
substance, and snake venom present in sufficient amounts to rapidly clot
said highly heparinized blood sample and to enable the serum to be
separated from said clotted matter, said serum to be chemically tested to
provide clinically accurate blood chemistry results.
20. A method of preparing a cocktail reagent for rapidly producing
serum from blood, comprising:
preparing a first solution by dissolving bovine thrombin in barbital
buffered saline to a concentration of 100 units per ml;
preparing a second solution by dissolving a snake venom in barbital
buffered saline to a concentration of 1 mg per ml;
preparing a third solution by dissolving protamine sulfate in a sodium
chloride solution to a concentration of 10 mg per ml; and
combining a predetermined amount of said first, said second and
said third solutions to form a mixture such that the final concentration of said
mixture contains 0.5 units thrombin per ml, 0.01 mg snake venom per ml,
and 0.05 mg protamine sulfate per ml.
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