Appeal No. 1997-1307
Application No. 08/014,012
Claim 23 is illustrative of the subject matter on appeal and is reproduced
below:
23. A process for producing cytidine diphosphate choline, which comprises
contacting an enzymatically active treatment product of a culture of a
microorganism which carries a recombinant DNA comprising a DNA
fragment containing genes encoding pyrG, CCT and CKI and a vector
(hereinafter referred to as Microorganism A1) and an enzymatically
active treatment product of a culture of another microorganism which has
orotate phosphoribosyltransferase, OMP decarboxylase,
nucleosidemonophosphate kinase and nucleosidediphosphate kinase
activities (hereinafter referred to as Microorganism B) with orotic acid
and choline in a liquid medium; allowing cytidine diphosphate choline to
accumulate in the liquid medium; and recovering cytidine diphosphate
choline from the liquid medium.
Claim 24 is substantially the same as claim 23 with two exceptions:
(1) Microorganism A1 is renamed Microorganism A2 and is not required to contain
the gene encoding CKI, and (2) “phosphorylcholine” is “in the liquid medium” instead
of “choline.”
The references relied upon by the examiner are:
Gennari 4,789,666 Dec. 6, 1988
Weng et al. (Weng), “Nucleotide Sequence of Escherichia coli pyrG Encoding CTP
Synthetase,” J. Biol. Chem., Vol. 261, No. 12, pp. 5568-5574 (1986)
Neuhard et. al. (Neuhard), Purines and Pyrimidines, in 1 Escherichia coli and
Salmonella Typhimurium: Cellular and Molecular Biology 445-473 (Frederick C.
Neidhardt et al., eds., 1987)
Hosaka et al. (Hosaka), “Cloning and Characterization of the Yeast CKI Gene
Encoding Choline Kinase and Its Expression in Escherichia coli,” J. Biol. Chem.,
Vol. 264, No. 4, pp. 2053-2059 (1989)
Nudler et al. (Nudler), “The derepression of enzymes of de novo pyrimidine
biosynthesis pathway in Brevibacterium ammoniagenes producing uridine-5-
monophosphate and uracil, FEMS Microbiology Letters, Vol. 82, pp. 263-266
(1991)
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