Appeal No. 1997-3221
Application No. 08/249,241
II. The EAA4 Subclass:
Appeal No. 1997-3221
Application No. 08/249,241
Claims 26, 27, 28, and 45 are illustrative of the subject matter on appeal and
are reproduced below:
26. A method of detecting interaction between a test ligand and a human
CNS receptor, which comprises the steps of incubating the test ligand
with a human EAA4 receptor-producing cell, or with a membrane
preparation derived from said cell, the cell having incorporated
expressibly therein a heterologous polynucleotide that encodes a human
EAA4 receptor selected from the group consisting of:
EAA4a having the amino acid sequence of amino acids 1-
877 of SEQ ID NO:2, and
EAA4b having the amino acid sequence of amino acids 1-
877 of SEQ ID NO:2 with the exception that the amino acid at
position 727 is aspartic acid,
and then measuring ligand-induced electrical current across said cell or
membrane.
27. A method according to claim 26, wherein the receptor-producing cell is
an EAA4a receptor-producing cell.
28. A method according to claim 27, wherein the cell is a mammalian cell.
45. A method according to claim 26, wherein the heterologous DNA has a
nucleotide sequence of nucleotides 226-2855 of SEQ ID NO:1 with the
exception that the guanosine at position 2403 is replace by adenosine.
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