Ex parte KAMBOJ et al.; Ex parte NUTT; Ex parte FOLDES et al. - Page 21


                         Appeal No.  1999-1393                                                                                                                            
                         Application No.  08/242,344                                                                                                                      
                         II.       The EAA4 Subclass:                                                                                                                     
                                                                    Appeal No. 1997-3221                                                                                  
                                                                 Application No. 08/249,241                                                                               
                                   Claims 26, 27, 28, and 45 are illustrative of the subject matter on appeal and                                                         
                         are reproduced below:                                                                                                                            
                                   26. A method of detecting interaction between a test ligand and a human                                                                
                                         CNS receptor, which comprises the steps of incubating the test ligand                                                            
                                         with a human EAA4 receptor-producing cell, or with a membrane                                                                    
                                         preparation derived from said cell, the cell having incorporated                                                                 
                                         expressibly therein a heterologous polynucleotide that encodes a human                                                           
                                         EAA4 receptor selected from the group consisting of:                                                                             
                                                       EAA4a having the amino acid sequence of amino acids      1-                                                        
                                                       877 of SEQ ID NO:2, and                                                                                            
                                                       EAA4b having the amino acid sequence of amino acids      1-                                                        
                                                       877 of SEQ ID NO:2 with the exception that the amino acid at                                                       
                                                       position 727 is aspartic acid,                                                                                     
                                         and then measuring ligand-induced electrical current across said cell or                                                         
                                         membrane.                                                                                                                        
                                   27.  A method according to claim 26, wherein the receptor-producing cell    is                                                         
                                         an EAA4a receptor-producing cell.                                                                                                
                                   28. A method according to claim 27, wherein the cell is a mammalian cell.                                                              
                                   45. A method according to claim 26, wherein the heterologous DNA has a                                                                 
                                         nucleotide sequence of nucleotides 226-2855 of SEQ ID NO:1 with the                                                              
                                         exception that the guanosine at position 2403 is replace by adenosine.                                                           













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